Construct: shRNA TRCN0000131120

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024782.1-937s1c1
DNA Barcode:
GCTGTCAAAGGTCAAGAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NHEJ1 (79840)
Gene Description:
non-homologous end joining factor 1
Transcript:
RefSeq NM_024782.1 (NON-CURRENT)
Match location:
Position 937 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000131120)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCAAAGGTCAAGAGGAA
Hairpin Sequence:
5'-CCGG-GCTGTCAAAGGTCAAGAGGAA-CTCGAG-TTCCTCTTGACCTTTGACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCAAAGGTCAAGAGGAACTCGAGTTCCTCTTGACCTTTGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCAAAGGTCAAGAGGAACTCGAGTTCCTCTTGACCTTTGACAGC-3'

Other clones with same target sequence:

(none)