Construct: shRNA TRCN0000131135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025132.2-3297s1c1
DNA Barcode:
GCTCGGAAGATAATGTGGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR19 (57728)
Gene Description:
WD repeat domain 19
Transcript:
RefSeq NM_025132.2 (NON-CURRENT)
Match location:
Position 3297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000131135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGGAAGATAATGTGGCAA
Hairpin Sequence:
5'-CCGG-GCTCGGAAGATAATGTGGCAA-CTCGAG-TTGCCACATTATCTTCCGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGGAAGATAATGTGGCAACTCGAGTTGCCACATTATCTTCCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGGAAGATAATGTGGCAACTCGAGTTGCCACATTATCTTCCGAGC-3'

Other clones with same target sequence:

(none)