Construct: shRNA TRCN0000133640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020234.3-761s1c1
DNA Barcode:
GAAGAACAAGAGTTCTGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DTWD1 (56986)
Gene Description:
DTW domain containing 1
Transcript:
RefSeq NM_020234.3 (NON-CURRENT)
Match location:
Position 761 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAACAAGAGTTCTGTGAT
Hairpin Sequence:
5'-CCGG-GAAGAACAAGAGTTCTGTGAT-CTCGAG-ATCACAGAACTCTTGTTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAACAAGAGTTCTGTGATCTCGAGATCACAGAACTCTTGTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAACAAGAGTTCTGTGATCTCGAGATCACAGAACTCTTGTTCTTC-3'

Other clones with same target sequence:

(none)