Construct: shRNA TRCN0000133663

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015534.3-3593s1c1
DNA Barcode:
GCACAGAAACTGACTTCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZZZ3 (26009)
Gene Description:
zinc finger ZZ-type containing 3
Transcript:
RefSeq NM_015534.3 (NON-CURRENT)
Match location:
Position 3593 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133663)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGAAACTGACTTCTTTA
Hairpin Sequence:
5'-CCGG-GCACAGAAACTGACTTCTTTA-CTCGAG-TAAAGAAGTCAGTTTCTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGAAACTGACTTCTTTACTCGAGTAAAGAAGTCAGTTTCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGAAACTGACTTCTTTACTCGAGTAAAGAAGTCAGTTTCTGTGC-3'

Other clones with same target sequence:

(none)