Construct: shRNA TRCN0000133675

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080874.2-1999s1c1
DNA Barcode:
GAGAACTTAATCGCACTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASB5 (140458)
Gene Description:
ankyrin repeat and SOCS box containing 5
Transcript:
RefSeq NM_080874.2 (NON-CURRENT)
Match location:
Position 1999 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133675)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAACTTAATCGCACTGTTT
Hairpin Sequence:
5'-CCGG-GAGAACTTAATCGCACTGTTT-CTCGAG-AAACAGTGCGATTAAGTTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAACTTAATCGCACTGTTTCTCGAGAAACAGTGCGATTAAGTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAACTTAATCGCACTGTTTCTCGAGAAACAGTGCGATTAAGTTCTC-3'

Other clones with same target sequence:

(none)