Construct: shRNA TRCN0000133715

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024587.2-321s1c1
DNA Barcode:
GCTGCTCTTTGATTATGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM53 (79639)
Gene Description:
transmembrane protein 53
Transcript:
RefSeq NM_024587.2 (NON-CURRENT)
Match location:
Position 321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133715)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTCTTTGATTATGAGAT
Hairpin Sequence:
5'-CCGG-GCTGCTCTTTGATTATGAGAT-CTCGAG-ATCTCATAATCAAAGAGCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTCTTTGATTATGAGATCTCGAGATCTCATAATCAAAGAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTCTTTGATTATGAGATCTCGAGATCTCATAATCAAAGAGCAGC-3'

Other clones with same target sequence:

(none)