Construct: shRNA TRCN0000133730

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025031.1-205s1c1
DNA Barcode:
CAAACTATCAAATGCAGAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C7orf69 (80099)
Gene Description:
chromosome 7 open reading frame 69
Transcript:
RefSeq NM_025031.1 (NON-CURRENT)
Match location:
Position 205 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133730)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACTATCAAATGCAGAGCA
Hairpin Sequence:
5'-CCGG-CAAACTATCAAATGCAGAGCA-CTCGAG-TGCTCTGCATTTGATAGTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACTATCAAATGCAGAGCACTCGAGTGCTCTGCATTTGATAGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACTATCAAATGCAGAGCACTCGAGTGCTCTGCATTTGATAGTTTG-3'

Other clones with same target sequence:

(none)