Construct: shRNA TRCN0000133737

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152731.1-1247s1c1
DNA Barcode:
CCAGTCAAATAAGCATGCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BEND6 (221336)
Gene Description:
BEN domain containing 6
Transcript:
RefSeq NM_152731.1 (NON-CURRENT)
Match location:
Position 1247 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133737)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCAAATAAGCATGCAAT
Hairpin Sequence:
5'-CCGG-CCAGTCAAATAAGCATGCAAT-CTCGAG-ATTGCATGCTTATTTGACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCAAATAAGCATGCAATCTCGAGATTGCATGCTTATTTGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCAAATAAGCATGCAATCTCGAGATTGCATGCTTATTTGACTGG-3'

Other clones with same target sequence:

(none)