Construct: shRNA TRCN0000133743

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005361.2-1313s1c1
DNA Barcode:
CATTGAAACCAGCTATGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGEA2 (4101)
Gene Description:
MAGE family member A2
Transcript:
RefSeq NM_005361.2 (NON-CURRENT)
Match location:
Position 1313 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133743)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTGAAACCAGCTATGTGAA
Hairpin Sequence:
5'-CCGG-CATTGAAACCAGCTATGTGAA-CTCGAG-TTCACATAGCTGGTTTCAATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTGAAACCAGCTATGTGAACTCGAGTTCACATAGCTGGTTTCAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTGAAACCAGCTATGTGAACTCGAGTTCACATAGCTGGTTTCAATG-3'

Other clones with same target sequence:

  1. TRCN0000152048