Construct: shRNA TRCN0000133751

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031455.2-1060s1c1
DNA Barcode:
CGCATTTGGTAGAGTCTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC3 (83643)
Gene Description:
coiled-coil domain containing 3
Transcript:
RefSeq NM_031455.2 (NON-CURRENT)
Match location:
Position 1060 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133751)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATTTGGTAGAGTCTAAAT
Hairpin Sequence:
5'-CCGG-CGCATTTGGTAGAGTCTAAAT-CTCGAG-ATTTAGACTCTACCAAATGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATTTGGTAGAGTCTAAATCTCGAGATTTAGACTCTACCAAATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATTTGGTAGAGTCTAAATCTCGAGATTTAGACTCTACCAAATGCG-3'

Other clones with same target sequence:

(none)