Construct: shRNA TRCN0000133830

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032222.1-295s1c1
DNA Barcode:
CAGATACTTTCTGGATCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MINDY4 (84182)
Gene Description:
MINDY lysine 48 deubiquitinase 4
Transcript:
RefSeq NM_032222.1 (NON-CURRENT)
Match location:
Position 295 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133830)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATACTTTCTGGATCACTT
Hairpin Sequence:
5'-CCGG-CAGATACTTTCTGGATCACTT-CTCGAG-AAGTGATCCAGAAAGTATCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATACTTTCTGGATCACTTCTCGAGAAGTGATCCAGAAAGTATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATACTTTCTGGATCACTTCTCGAGAAGTGATCCAGAAAGTATCTG-3'

Other clones with same target sequence:

(none)