Construct: shRNA TRCN0000133888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_370981.1-99s1c1
DNA Barcode:
CATGTCCTTGTGGTTACATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C16orf87 (388272)
Gene Description:
chromosome 16 open reading frame 87
Transcript:
RefSeq XM_370981.1 (NON-CURRENT)
Match location:
Position 99 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGTCCTTGTGGTTACATAT
Hairpin Sequence:
5'-CCGG-CATGTCCTTGTGGTTACATAT-CTCGAG-ATATGTAACCACAAGGACATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGTCCTTGTGGTTACATATCTCGAGATATGTAACCACAAGGACATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGTCCTTGTGGTTACATATCTCGAGATATGTAACCACAAGGACATG-3'

Other clones with same target sequence:

(none)