Construct: shRNA TRCN0000133939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013740.1-3176s1c1
DNA Barcode:
CGTATGGTCCAAATTCAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC442601 (442601)
Gene Description:
LOC442601
Transcript:
RefSeq NM_001013740.1 (NON-CURRENT)
Match location:
Position 3176 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTATGGTCCAAATTCAGATT
Hairpin Sequence:
5'-CCGG-CGTATGGTCCAAATTCAGATT-CTCGAG-AATCTGAATTTGGACCATACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTATGGTCCAAATTCAGATTCTCGAGAATCTGAATTTGGACCATACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTATGGTCCAAATTCAGATTCTCGAGAATCTGAATTTGGACCATACG-3'

Other clones with same target sequence:

(none)