Construct: shRNA TRCN0000133970

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013688.1-1508s1c1
DNA Barcode:
CCATCAACAGATGAATGGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC401620 (401620)
Gene Description:
LINE-1 reverse transcriptase homolog
Transcript:
RefSeq NM_001013688.1 (NON-CURRENT)
Match location:
Position 1508 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133970)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCAACAGATGAATGGATA
Hairpin Sequence:
5'-CCGG-CCATCAACAGATGAATGGATA-CTCGAG-TATCCATTCATCTGTTGATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCAACAGATGAATGGATACTCGAGTATCCATTCATCTGTTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCAACAGATGAATGGATACTCGAGTATCCATTCATCTGTTGATGG-3'

Other clones with same target sequence:

(none)