Construct: shRNA TRCN0000133983

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080667.3-719s1c1
DNA Barcode:
CTTGAACACGAAGAGATGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFAP36 (112942)
Gene Description:
cilia and flagella associated protein 36
Transcript:
RefSeq NM_080667.3 (NON-CURRENT)
Match location:
Position 719 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133983)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGAACACGAAGAGATGAAA
Hairpin Sequence:
5'-CCGG-CTTGAACACGAAGAGATGAAA-CTCGAG-TTTCATCTCTTCGTGTTCAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGAACACGAAGAGATGAAACTCGAGTTTCATCTCTTCGTGTTCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGAACACGAAGAGATGAAACTCGAGTTTCATCTCTTCGTGTTCAAG-3'

Other clones with same target sequence:

  1. TRCN0000343417