Construct: shRNA TRCN0000133989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005452.4-922s1c1
DNA Barcode:
CTTCAGAAACAGGGTTTCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR46 (9277)
Gene Description:
WD repeat domain 46
Transcript:
RefSeq NM_005452.4 (NON-CURRENT)
Match location:
Position 922 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAGAAACAGGGTTTCTAA
Hairpin Sequence:
5'-CCGG-CTTCAGAAACAGGGTTTCTAA-CTCGAG-TTAGAAACCCTGTTTCTGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAGAAACAGGGTTTCTAACTCGAGTTAGAAACCCTGTTTCTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAGAAACAGGGTTTCTAACTCGAGTTAGAAACCCTGTTTCTGAAG-3'

Other clones with same target sequence:

(none)