Construct: shRNA TRCN0000134066

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023927.1-1173s1c1
DNA Barcode:
GTCAGAAACTGTTGGAATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRAMD2B (65983)
Gene Description:
GRAM domain containing 2B
Transcript:
RefSeq NM_023927.1 (NON-CURRENT)
Match location:
Position 1173 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134066)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGAAACTGTTGGAATCTT
Hairpin Sequence:
5'-CCGG-GTCAGAAACTGTTGGAATCTT-CTCGAG-AAGATTCCAACAGTTTCTGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGAAACTGTTGGAATCTTCTCGAGAAGATTCCAACAGTTTCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGAAACTGTTGGAATCTTCTCGAGAAGATTCCAACAGTTTCTGAC-3'

Other clones with same target sequence:

(none)