Construct: shRNA TRCN0000134121

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024613.2-1105s1c1
DNA Barcode:
CCTGAGAAACTTGTAACCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEKHF2 (79666)
Gene Description:
pleckstrin homology and FYVE domain containing 2
Transcript:
RefSeq NM_024613.2 (NON-CURRENT)
Match location:
Position 1105 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134121)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGAAACTTGTAACCTAT
Hairpin Sequence:
5'-CCGG-CCTGAGAAACTTGTAACCTAT-CTCGAG-ATAGGTTACAAGTTTCTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGAAACTTGTAACCTATCTCGAGATAGGTTACAAGTTTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGAAACTTGTAACCTATCTCGAGATAGGTTACAAGTTTCTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000276218