Construct: shRNA TRCN0000134156

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178823.2-752s1c1
DNA Barcode:
CAGCATATTGATTACCAGCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFAP206 (154313)
Gene Description:
cilia and flagella associated protein 206
Transcript:
RefSeq NM_178823.2 (NON-CURRENT)
Match location:
Position 752 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134156)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCATATTGATTACCAGCTT
Hairpin Sequence:
5'-CCGG-CAGCATATTGATTACCAGCTT-CTCGAG-AAGCTGGTAATCAATATGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCATATTGATTACCAGCTTCTCGAGAAGCTGGTAATCAATATGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCATATTGATTACCAGCTTCTCGAGAAGCTGGTAATCAATATGCTG-3'

Other clones with same target sequence:

(none)