Construct: shRNA TRCN0000134193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024726.3-2549s1c1
DNA Barcode:
CTTTAAGAACTGGTATGCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IQCA1 (79781)
Gene Description:
IQ motif containing with AAA domain 1
Transcript:
RefSeq NM_024726.3 (NON-CURRENT)
Match location:
Position 2549 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTAAGAACTGGTATGCCAA
Hairpin Sequence:
5'-CCGG-CTTTAAGAACTGGTATGCCAA-CTCGAG-TTGGCATACCAGTTCTTAAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTAAGAACTGGTATGCCAACTCGAGTTGGCATACCAGTTCTTAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTAAGAACTGGTATGCCAACTCGAGTTGGCATACCAGTTCTTAAAG-3'

Other clones with same target sequence:

(none)