Construct: shRNA TRCN0000134262

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001007560.1-945s1c1
DNA Barcode:
GAGGAGAAACTCAGATACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LINC00523 (283601)
Gene Description:
long intergenic non-protein coding RNA 523
Transcript:
RefSeq NM_001007560.1 (NON-CURRENT)
Match location:
Position 945 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134262)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGAAACTCAGATACATA
Hairpin Sequence:
5'-CCGG-GAGGAGAAACTCAGATACATA-CTCGAG-TATGTATCTGAGTTTCTCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGAAACTCAGATACATACTCGAGTATGTATCTGAGTTTCTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGAAACTCAGATACATACTCGAGTATGTATCTGAGTTTCTCCTC-3'

Other clones with same target sequence:

(none)