Construct: shRNA TRCN0000134305

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_113708.5-1591s1c1
DNA Barcode:
GCCACATCCATTCATTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AMN1 (196394)
Gene Description:
antagonist of mitotic exit network 1 homolog
Transcript:
RefSeq XM_113708.5 (NON-CURRENT)
Match location:
Position 1591 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134305)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACATCCATTCATTGATTT
Hairpin Sequence:
5'-CCGG-GCCACATCCATTCATTGATTT-CTCGAG-AAATCAATGAATGGATGTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACATCCATTCATTGATTTCTCGAGAAATCAATGAATGGATGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACATCCATTCATTGATTTCTCGAGAAATCAATGAATGGATGTGGC-3'

Other clones with same target sequence:

(none)