Construct: shRNA TRCN0000134323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017847.2-794s1c1
DNA Barcode:
GAGGAAATACTCAAGCAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ODR4 (54953)
Gene Description:
odr-4 GPCR localization factor homolog
Transcript:
RefSeq NM_017847.2 (NON-CURRENT)
Match location:
Position 794 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAAATACTCAAGCAACTA
Hairpin Sequence:
5'-CCGG-GAGGAAATACTCAAGCAACTA-CTCGAG-TAGTTGCTTGAGTATTTCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAAATACTCAAGCAACTACTCGAGTAGTTGCTTGAGTATTTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAAATACTCAAGCAACTACTCGAGTAGTTGCTTGAGTATTTCCTC-3'

Other clones with same target sequence:

  1. TRCN0000330981