Construct: shRNA TRCN0000134403

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152543.1-396s1c1
DNA Barcode:
GTCAAGCTTCTCTTGATTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C4orf45 (152940)
Gene Description:
chromosome 4 open reading frame 45
Transcript:
RefSeq NM_152543.1 (NON-CURRENT)
Match location:
Position 396 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134403)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAAGCTTCTCTTGATTCAA
Hairpin Sequence:
5'-CCGG-GTCAAGCTTCTCTTGATTCAA-CTCGAG-TTGAATCAAGAGAAGCTTGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAAGCTTCTCTTGATTCAACTCGAGTTGAATCAAGAGAAGCTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAAGCTTCTCTTGATTCAACTCGAGTTGAATCAAGAGAAGCTTGAC-3'

Other clones with same target sequence:

(none)