Construct: shRNA TRCN0000134499

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032439.1-2198s1c1
DNA Barcode:
GCCTCTGAAATAAGTCTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHYHIPL (84457)
Gene Description:
phytanoyl-CoA 2-hydroxylase interacting protein like
Transcript:
RefSeq NM_032439.1 (NON-CURRENT)
Match location:
Position 2198 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134499)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTGAAATAAGTCTGTTT
Hairpin Sequence:
5'-CCGG-GCCTCTGAAATAAGTCTGTTT-CTCGAG-AAACAGACTTATTTCAGAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTGAAATAAGTCTGTTTCTCGAGAAACAGACTTATTTCAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTGAAATAAGTCTGTTTCTCGAGAAACAGACTTATTTCAGAGGC-3'

Other clones with same target sequence:

(none)