Construct: shRNA TRCN0000134579

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015026.1-5561s1c1
DNA Barcode:
GAAAGCAGGAAGATAGTCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MON2 (23041)
Gene Description:
MON2 homolog, regulator of endosome-to-Golgi trafficking
Transcript:
RefSeq NM_015026.1 (NON-CURRENT)
Match location:
Position 5561 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134579)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGCAGGAAGATAGTCTAA
Hairpin Sequence:
5'-CCGG-GAAAGCAGGAAGATAGTCTAA-CTCGAG-TTAGACTATCTTCCTGCTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGCAGGAAGATAGTCTAACTCGAGTTAGACTATCTTCCTGCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGCAGGAAGATAGTCTAACTCGAGTTAGACTATCTTCCTGCTTTC-3'

Other clones with same target sequence:

(none)