Construct: shRNA TRCN0000134581

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018474.2-202s1c1
DNA Barcode:
GTCTGATACATGCAGAGTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIZ (55857)
Gene Description:
kizuna centrosomal protein
Transcript:
RefSeq NM_018474.2 (NON-CURRENT)
Match location:
Position 202 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134581)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGATACATGCAGAGTTAA
Hairpin Sequence:
5'-CCGG-GTCTGATACATGCAGAGTTAA-CTCGAG-TTAACTCTGCATGTATCAGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGATACATGCAGAGTTAACTCGAGTTAACTCTGCATGTATCAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGATACATGCAGAGTTAACTCGAGTTAACTCTGCATGTATCAGAC-3'

Other clones with same target sequence:

(none)