Construct: shRNA TRCN0000134603

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015380.3-219s1c1
DNA Barcode:
GATTAGGAGAAGAAGCTGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAMM50 (25813)
Gene Description:
SAMM50 sorting and assembly machinery component
Transcript:
RefSeq NM_015380.3 (NON-CURRENT)
Match location:
Position 219 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134603)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTAGGAGAAGAAGCTGAAT
Hairpin Sequence:
5'-CCGG-GATTAGGAGAAGAAGCTGAAT-CTCGAG-ATTCAGCTTCTTCTCCTAATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTAGGAGAAGAAGCTGAATCTCGAGATTCAGCTTCTTCTCCTAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTAGGAGAAGAAGCTGAATCTCGAGATTCAGCTTCTTCTCCTAATC-3'

Other clones with same target sequence:

(none)