Construct: shRNA TRCN0000134634

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033364.2-482s1c1
DNA Barcode:
GTTACTGGAAAGAATCGCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAATS1 (89876)
Gene Description:
MYCBP associated and testis expressed 1
Transcript:
RefSeq NM_033364.2 (NON-CURRENT)
Match location:
Position 482 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134634)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTACTGGAAAGAATCGCTAT
Hairpin Sequence:
5'-CCGG-GTTACTGGAAAGAATCGCTAT-CTCGAG-ATAGCGATTCTTTCCAGTAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTACTGGAAAGAATCGCTATCTCGAGATAGCGATTCTTTCCAGTAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTACTGGAAAGAATCGCTATCTCGAGATAGCGATTCTTTCCAGTAAC-3'

Other clones with same target sequence:

(none)