Construct: shRNA TRCN0000134727

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015196.2-2796s1c1
DNA Barcode:
GCAAGAACTTTCTCGATACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM131L (23240)
Gene Description:
transmembrane 131 like
Transcript:
RefSeq NM_015196.2 (NON-CURRENT)
Match location:
Position 2796 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134727)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGAACTTTCTCGATACAT
Hairpin Sequence:
5'-CCGG-GCAAGAACTTTCTCGATACAT-CTCGAG-ATGTATCGAGAAAGTTCTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGAACTTTCTCGATACATCTCGAGATGTATCGAGAAAGTTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGAACTTTCTCGATACATCTCGAGATGTATCGAGAAAGTTCTTGC-3'

Other clones with same target sequence:

(none)