Construct: shRNA TRCN0000134737

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004968.1-346s1c1
DNA Barcode:
GCTAGAGCTGTTTCATTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ICA1 (3382)
Gene Description:
islet cell autoantigen 1
Transcript:
RefSeq NM_004968.1 (NON-CURRENT)
Match location:
Position 346 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134737)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGAGCTGTTTCATTCAAT
Hairpin Sequence:
5'-CCGG-GCTAGAGCTGTTTCATTCAAT-CTCGAG-ATTGAATGAAACAGCTCTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGAGCTGTTTCATTCAATCTCGAGATTGAATGAAACAGCTCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGAGCTGTTTCATTCAATCTCGAGATTGAATGAAACAGCTCTAGC-3'

Other clones with same target sequence:

  1. TRCN0000276051