Construct: shRNA TRCN0000134860

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012992.1-1670s1c1
DNA Barcode:
CCAGTAAACTTAAGCCTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C1orf136 (400574)
Gene Description:
chromosome 1 open reading frame 136
Transcript:
RefSeq NM_001012992.1 (NON-CURRENT)
Match location:
Position 1670 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134860)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTAAACTTAAGCCTTCAT
Hairpin Sequence:
5'-CCGG-CCAGTAAACTTAAGCCTTCAT-CTCGAG-ATGAAGGCTTAAGTTTACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTAAACTTAAGCCTTCATCTCGAGATGAAGGCTTAAGTTTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTAAACTTAAGCCTTCATCTCGAGATGAAGGCTTAAGTTTACTGG-3'

Other clones with same target sequence:

(none)