Construct: shRNA TRCN0000134866

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016030.5-2132s1c1
DNA Barcode:
CTTCACAGAGATCTTAAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRAPPC12 (51112)
Gene Description:
trafficking protein particle complex 12
Transcript:
RefSeq NM_016030.5 (NON-CURRENT)
Match location:
Position 2132 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000134866)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCACAGAGATCTTAAGGAT
Hairpin Sequence:
5'-CCGG-CTTCACAGAGATCTTAAGGAT-CTCGAG-ATCCTTAAGATCTCTGTGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCACAGAGATCTTAAGGATCTCGAGATCCTTAAGATCTCTGTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCACAGAGATCTTAAGGATCTCGAGATCCTTAAGATCTCTGTGAAG-3'

Other clones with same target sequence:

(none)