Construct: shRNA TRCN0000135003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144590.1-504s1c1
DNA Barcode:
CTTGTACGAATGCTACTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD22 (118932)
Gene Description:
ankyrin repeat domain 22
Transcript:
RefSeq NM_144590.1 (NON-CURRENT)
Match location:
Position 504 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGTACGAATGCTACTTGAT
Hairpin Sequence:
5'-CCGG-CTTGTACGAATGCTACTTGAT-CTCGAG-ATCAAGTAGCATTCGTACAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGTACGAATGCTACTTGATCTCGAGATCAAGTAGCATTCGTACAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGTACGAATGCTACTTGATCTCGAGATCAAGTAGCATTCGTACAAG-3'

Other clones with same target sequence:

(none)