Construct: shRNA TRCN0000135123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024949.3-2293s1c1
DNA Barcode:
CTAGACTTAGAACTGGACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WWC2 (80014)
Gene Description:
WW and C2 domain containing 2
Transcript:
RefSeq NM_024949.3 (NON-CURRENT)
Match location:
Position 2293 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGACTTAGAACTGGACCTT
Hairpin Sequence:
5'-CCGG-CTAGACTTAGAACTGGACCTT-CTCGAG-AAGGTCCAGTTCTAAGTCTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGACTTAGAACTGGACCTTCTCGAGAAGGTCCAGTTCTAAGTCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGACTTAGAACTGGACCTTCTCGAGAAGGTCCAGTTCTAAGTCTAG-3'

Other clones with same target sequence:

(none)