Construct: shRNA TRCN0000135232

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032856.1-1339s1c1
DNA Barcode:
CGAAGGTTAGAAGAAACGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR73 (84942)
Gene Description:
WD repeat domain 73
Transcript:
RefSeq NM_032856.1 (NON-CURRENT)
Match location:
Position 1339 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135232)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGGTTAGAAGAAACGCAT
Hairpin Sequence:
5'-CCGG-CGAAGGTTAGAAGAAACGCAT-CTCGAG-ATGCGTTTCTTCTAACCTTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGGTTAGAAGAAACGCATCTCGAGATGCGTTTCTTCTAACCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGGTTAGAAGAAACGCATCTCGAGATGCGTTTCTTCTAACCTTCG-3'

Other clones with same target sequence:

  1. TRCN0000330900