Construct: shRNA TRCN0000135387

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152434.1-247s1c1
DNA Barcode:
GAACAGTCCAAACTGATGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CWF19L2 (143884)
Gene Description:
CWF19 like cell cycle control factor 2
Transcript:
RefSeq NM_152434.1 (NON-CURRENT)
Match location:
Position 247 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135387)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACAGTCCAAACTGATGGAA
Hairpin Sequence:
5'-CCGG-GAACAGTCCAAACTGATGGAA-CTCGAG-TTCCATCAGTTTGGACTGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACAGTCCAAACTGATGGAACTCGAGTTCCATCAGTTTGGACTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACAGTCCAAACTGATGGAACTCGAGTTCCATCAGTTTGGACTGTTC-3'

Other clones with same target sequence:

(none)