Construct: shRNA TRCN0000135396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018201.2-352s1c1
DNA Barcode:
GAACACGTACTTCAAGGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBC1D13 (54662)
Gene Description:
TBC1 domain family member 13
Transcript:
RefSeq NM_018201.2 (NON-CURRENT)
Match location:
Position 352 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACACGTACTTCAAGGACAA
Hairpin Sequence:
5'-CCGG-GAACACGTACTTCAAGGACAA-CTCGAG-TTGTCCTTGAAGTACGTGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACACGTACTTCAAGGACAACTCGAGTTGTCCTTGAAGTACGTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACACGTACTTCAAGGACAACTCGAGTTGTCCTTGAAGTACGTGTTC-3'

Other clones with same target sequence:

(none)