Construct: shRNA TRCN0000135435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152732.2-447s1c1
DNA Barcode:
GTGTCTGTCATTGACCAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSPH9 (221421)
Gene Description:
radial spoke head component 9
Transcript:
RefSeq NM_152732.2 (NON-CURRENT)
Match location:
Position 447 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTCTGTCATTGACCAGATT
Hairpin Sequence:
5'-CCGG-GTGTCTGTCATTGACCAGATT-CTCGAG-AATCTGGTCAATGACAGACAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTCTGTCATTGACCAGATTCTCGAGAATCTGGTCAATGACAGACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTCTGTCATTGACCAGATTCTCGAGAATCTGGTCAATGACAGACAC-3'

Other clones with same target sequence:

(none)