Construct: shRNA TRCN0000135444
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_024874.3-3858s1c2
- DNA Barcode:
- GCTGAGATGGTAGCATAGAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KIAA0319L (79932)
- Gene Description:
- KIAA0319 like
- Transcript:
- RefSeq NM_024874.3 (NON-CURRENT)
- Match location:
- Position 3858 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000135444)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTGAGATGGTAGCATAGAAA
- Hairpin Sequence:
-
5'-CCGG-GCTGAGATGGTAGCATAGAAA-CTCGAG-TTTCTATGCTACCATCTCAGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGAGATGGTAGCATAGAAACTCGAGTTTCTATGCTACCATCTCAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGAGATGGTAGCATAGAAACTCGAGTTTCTATGCTACCATCTCAGC-3'
Other clones with same target sequence:
-
TRCN0000299018
-
TRCN0000123109
