Construct: shRNA TRCN0000135454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015534.3-2167s1c1
DNA Barcode:
GAGATCGTATGGGACCAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZZZ3 (26009)
Gene Description:
zinc finger ZZ-type containing 3
Transcript:
RefSeq NM_015534.3 (NON-CURRENT)
Match location:
Position 2167 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATCGTATGGGACCAATAT
Hairpin Sequence:
5'-CCGG-GAGATCGTATGGGACCAATAT-CTCGAG-ATATTGGTCCCATACGATCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATCGTATGGGACCAATATCTCGAGATATTGGTCCCATACGATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATCGTATGGGACCAATATCTCGAGATATTGGTCCCATACGATCTC-3'

Other clones with same target sequence:

(none)