Construct: shRNA TRCN0000135493

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032434.2-1201s1c1
DNA Barcode:
GTACCTGATGATCGAAAGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF512 (84450)
Gene Description:
zinc finger protein 512
Transcript:
RefSeq NM_032434.2 (NON-CURRENT)
Match location:
Position 1201 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135493)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACCTGATGATCGAAAGTTA
Hairpin Sequence:
5'-CCGG-GTACCTGATGATCGAAAGTTA-CTCGAG-TAACTTTCGATCATCAGGTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACCTGATGATCGAAAGTTACTCGAGTAACTTTCGATCATCAGGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACCTGATGATCGAAAGTTACTCGAGTAACTTTCGATCATCAGGTAC-3'

Other clones with same target sequence:

(none)