Construct: shRNA TRCN0000135500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173556.2-1857s1c1
DNA Barcode:
GCTGCAAAGGAAACACAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC83 (220047)
Gene Description:
coiled-coil domain containing 83
Transcript:
RefSeq NM_173556.2 (NON-CURRENT)
Match location:
Position 1857 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCAAAGGAAACACAACTT
Hairpin Sequence:
5'-CCGG-GCTGCAAAGGAAACACAACTT-CTCGAG-AAGTTGTGTTTCCTTTGCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCAAAGGAAACACAACTTCTCGAGAAGTTGTGTTTCCTTTGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCAAAGGAAACACAACTTCTCGAGAAGTTGTGTTTCCTTTGCAGC-3'

Other clones with same target sequence:

(none)