Construct: shRNA TRCN0000135520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_086905.7-80s1c1
DNA Barcode:
GAGTGTTTGAGTGTGAACTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDPF1 (150383)
Gene Description:
cysteine rich DPF motif domain containing 1
Transcript:
RefSeq XM_086905.7 (NON-CURRENT)
Match location:
Position 80 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGTTTGAGTGTGAACTCT
Hairpin Sequence:
5'-CCGG-GAGTGTTTGAGTGTGAACTCT-CTCGAG-AGAGTTCACACTCAAACACTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGTTTGAGTGTGAACTCTCTCGAGAGAGTTCACACTCAAACACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGTTTGAGTGTGAACTCTCTCGAGAGAGTTCACACTCAAACACTC-3'

Other clones with same target sequence:

(none)