Construct: shRNA TRCN0000135549

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031485.2-583s1c1
DNA Barcode:
GAGGAAGATGAAGAGGATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRWD1 (83743)
Gene Description:
glutamate rich WD repeat containing 1
Transcript:
RefSeq NM_031485.2 (NON-CURRENT)
Match location:
Position 583 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135549)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAAGATGAAGAGGATGAA
Hairpin Sequence:
5'-CCGG-GAGGAAGATGAAGAGGATGAA-CTCGAG-TTCATCCTCTTCATCTTCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAAGATGAAGAGGATGAACTCGAGTTCATCCTCTTCATCTTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAAGATGAAGAGGATGAACTCGAGTTCATCCTCTTCATCTTCCTC-3'

Other clones with same target sequence:

(none)