Construct: shRNA TRCN0000135553

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178350.1-961s1c1
DNA Barcode:
GACTCCATCAAAGATGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDFY1 (57590)
Gene Description:
WD repeat and FYVE domain containing 1
Transcript:
RefSeq NM_178350.1 (NON-CURRENT)
Match location:
Position 961 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135553)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCCATCAAAGATGAAGAT
Hairpin Sequence:
5'-CCGG-GACTCCATCAAAGATGAAGAT-CTCGAG-ATCTTCATCTTTGATGGAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCCATCAAAGATGAAGATCTCGAGATCTTCATCTTTGATGGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCCATCAAAGATGAAGATCTCGAGATCTTCATCTTTGATGGAGTC-3'

Other clones with same target sequence:

(none)