Construct: shRNA TRCN0000135646

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182529.1-1179s1c1
DNA Barcode:
GACATTGAAGACTCCTTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THAP5 (168451)
Gene Description:
THAP domain containing 5
Transcript:
RefSeq NM_182529.1 (NON-CURRENT)
Match location:
Position 1179 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135646)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACATTGAAGACTCCTTGTAT
Hairpin Sequence:
5'-CCGG-GACATTGAAGACTCCTTGTAT-CTCGAG-ATACAAGGAGTCTTCAATGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACATTGAAGACTCCTTGTATCTCGAGATACAAGGAGTCTTCAATGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACATTGAAGACTCCTTGTATCTCGAGATACAAGGAGTCTTCAATGTC-3'

Other clones with same target sequence:

(none)