Construct: shRNA TRCN0000135701
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_145290.2-4283s1c1
- DNA Barcode:
- CCTTGAAGCTATGAGCATTTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ADGRA3 (166647)
- Gene Description:
- adhesion G protein-coupled receptor A3
- Transcript:
- RefSeq NM_145290.2 (NON-CURRENT)
- Match location:
- Position 4283 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000135701)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTTGAAGCTATGAGCATTTA
- Hairpin Sequence:
-
5'-CCGG-CCTTGAAGCTATGAGCATTTA-CTCGAG-TAAATGCTCATAGCTTCAAGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTTGAAGCTATGAGCATTTACTCGAGTAAATGCTCATAGCTTCAAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTTGAAGCTATGAGCATTTACTCGAGTAAATGCTCATAGCTTCAAGG-3'
Other clones with same target sequence:
(none)
