Construct: shRNA TRCN0000135804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018093.1-466s1c1
DNA Barcode:
CTCATCACATGTGTGGATTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR74 (54663)
Gene Description:
WD repeat domain 74
Transcript:
RefSeq NM_018093.1 (NON-CURRENT)
Match location:
Position 466 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000135804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATCACATGTGTGGATTCT
Hairpin Sequence:
5'-CCGG-CTCATCACATGTGTGGATTCT-CTCGAG-AGAATCCACACATGTGATGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATCACATGTGTGGATTCTCTCGAGAGAATCCACACATGTGATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATCACATGTGTGGATTCTCTCGAGAGAATCCACACATGTGATGAG-3'

Other clones with same target sequence:

(none)