Construct: shRNA TRCN0000135838
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_014631.2-8258s1c1
- DNA Barcode:
- CGGTAGAGAAAGCAACAGAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SH3PXD2A (9644)
- Gene Description:
- SH3 and PX domains 2A
- Transcript:
- RefSeq NM_014631.2 (CURRENT)
- Match location:
- Position 8258 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000135838)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGGTAGAGAAAGCAACAGAAT
- Hairpin Sequence:
-
5'-CCGG-CGGTAGAGAAAGCAACAGAAT-CTCGAG-ATTCTGTTGCTTTCTCTACCG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGGTAGAGAAAGCAACAGAATCTCGAGATTCTGTTGCTTTCTCTACCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGGTAGAGAAAGCAACAGAATCTCGAGATTCTGTTGCTTTCTCTACCG-3'
Other clones with same target sequence:
(none)
